Tap Water Microbial Contamination in Prayagraj: Isolation, Purification and Characterization of Pathogenic Bacteria and Their Antibiotic Susceptibility Patterns

Authors

  • Aanchal Eusebius Amity Institute of Environmental Sciences, Amity University, Uttar Pradesh, 201303, India
  • M.P. Raju Amity Institute of Environmental Sciences, Amity University, Uttar Pradesh, 201303, India
  • Kartikeya Shukla Amity Institute of Environmental Sciences, Amity University, Uttar Pradesh, 201303, India
  • Priyanka Singh Centre of Food Technology, University of Allahabad, Prayagraj, Uttar Pradesh 211002, India

DOI:

https://doi.org/10.55863/ijees.2024.0347

Keywords:

Microbial characterization, Physicochemical analysis, Antibiotic resistance

Abstract

Since water is necessary for life, it is crucial for preserving human health and biological processes. However, serious health dangers are associated with drinking water contamination, especially in areas where access to clean water is scarce. The purpose of this study was to assess the physicochemical and microbiological properties of tap water from 10 distinct locations in Prayagraj, Uttar Pradesh, India. Water samples were collected and analyzed microbiologically to isolate and identify harmful bacteria. The study detected the presence of several gram-negative bacteria, including Leminorella grimontii, Morganella morganii, and Citrobacter braakii, among others. Temperature and pH levels varied throughout the sampling sites, according to physicochemical analysis, and some samples had levels of alkalinity over the WHO-recommended safe limits. Antibiotic susceptibility tests indicated that the isolated bacterial strains displayed varying levels of resistance to different antibiotics, with higher resistance to Gentamicin than to Ampicillin. These findings highlight the significance of ongoing water quality monitoring and improvement to reduce the risk of waterborne illnesses, particularly in areas with poor access to clean water and sanitation. Ensuring the safety and purity of water for human consumption is crucial for protecting public health, especially in areas vulnerable to illnesses caused by contaminated water. This study provides valuable insights into the microbiological ecology of Prayagraj tap water and emphasizes the need for effective water management strategies to protect human health and welfare.

References

Abbott, S.L., Moler, S., Green, N., Tran, R.K., Wainwright, K. and Janda, J.M. 2011. Clinical and laboratory diagnostic characteristics and cytotoxigenic potential of Hafnia alvei and Hafnia paralvei strains. Journal of Clinical Microbiology, 49, 3122-3126. https://doi.org/10.1128/JCM.00866-11

Athauda, I.D. 2023. Enhanced bactericidal effects and drug delivery with gentamicin-conjugated nanoparticles. Journal of Cluster Science, 35, 371-390.

Bhumbla, U. 2020. A study of isolation and identification of bacteria from lake water in and around Udaipur, Rajasthan. Journal of Family Medicine and Primary Care, 9, 751-754.

Blekher, L., Siegman-Igra, Y., Schwartz, D., Berger, S.A. and Carmeli, Y. 2000. Clinical significance and antibiotic resistance patterns of Leminorella spp., an emerging nosocomial pathogen. Journal of Clinical Microbiology, 38, 3036-3038. https://doi.org/10.1128/JCM.38.8.3036-3038.2000

Bonacina, L., Fasano, F., Mezzanotte, V. and Fornaroli, R. 2022. Effects of water temperature on freshwater macroinvertebrates: A systematic review. Biological Reviews, 98, 191-221. https://doi.org/10.1111/brv.12903

Boone, D.R. and Castenholz, R.W. 2021. Bergey’s Manual of Systematic Bacteriology. Springer, Cham. 721 pages

Chauhan, A. and Jindal, T. 2020. Biochemical and molecular methods for bacterial identification. pp. 425-268. In: Chauhan, A. and Jindal, T. (Eds.). Microbiological Methods for Environment, Food and Pharmaceutical Analysis. Springer, Cham. https://doi.org/10.1007/978-3-030-52024-3_10

Coelho, M.T.P., Barreto, E., Rangel, T.F., Diniz-Filho, J.A.F., Wuest, R.O., Bach, W., Skeels, A. McFadden, I.R., Roberts, D.W., Pellissier, L., Zimmermann, N.E. and Graham, C.H. 2023. The geography of climate and the global patterns of species diversity. Nature, 622, 537-544. https://doi.org/10.1038/s41586-023-06577-5

Du Plessis, A. 2022. Persistent degradation: Global water quality challenges and required actions. One Earth, 5, 129-131. https://doi.org/10.1016/j.oneear.2022.01.005

Fong, I.W., 2017. Animals and Mechanisms of Disease Transmission. pp. 15-38. In: Fong, I.W. (Eds.). Emerging Zoonoses: A Worldwide Perspective. Springer International Publishing, Cham. https://doi.org/10.1007/978-3-319-50890-0_2

Frenkel-Pinter, M., Rajaei, V., Glass, J.B., Hud, N.V. and Williams, L.D. 2021. Water and life: The medium is the message. Journal of Molecular Evolution, 89, 2-11. https://doi.org/10.1007/s00239-020-09978-6

Gabril, E.M.A. 2019. Site suitability analysis for urban development using geospatial technologies and AHP: A case study in Prayagraj, Uttar Pradesh, India. Pharma Innovation Journal, 8, 676-681.

Gomathi, S. 2018. WASH (Water, Sanitation and Hygiene). International Journal of Trends in Scientific Research and Development, 1(2), 576-579. http://www.ijtsrd.com/papers/ijtsrd7012.pdf

Gonçalves, J., Díaz, I., Torres-Franco, A., Rodríguez, E., Da Silva, P.G., Mesquita, J.R., Muñoz, R. and Garcia-Encina, P.A. 2023. Microbial contamination of environmental waters and wastewater: Detection methods and treatment technologies. pp. 461-483. In: Shah, M.P. (Ed.), Modern Approaches in Waste Bioremediation. Springer International Publishing, Cham. https://doi.org/10.1007/978-3-031-24086-7_22

Igwebuike, C., Yaglom, J., Huiting, L., Feng, H., Campbell, J.D., Wang, Z., Havasi, A., Pimentel, D., Sherman, M.Y. and Borkan, S.C. 2020. Cross organelle stress response disruption promotes gentamicin-induced proteotoxicity. Cell Death and Disease, 11, 217. https://doi.org/10.1038/s41419-020-2382-7

Jones, E. and Brown, D. 2020. Regional patterns of temperature variability. Journal of Climatology, 35, 210-225.

Kamal, I. and Hashmi, H. 2021. Physico-chemical and bacteriological analysis of tap water of a residential university of Pakistan. International Research Journal of Environmental Sciences, 10, 15-29.

Khan, W.A., Ali, S. and Shah, S.A. 2022. Water pollution: Sources and its impact on human health, control and managing. Journal of International Cooperation and Development, 5, 69. https://doi.org/10.36941/jicd-2022-0005

Kristanti, R.A., Hadibarata, T., Syafrudin, M., Yilmaz, M. and Abdullah, S. 2022. Microbiological contaminants in drinking water: Current status and challenges. International Journal of Environmental Pollution, 233, 299. https://doi.org/10.1007/s11270-022-05698-3

Lee, R., Choi, S.-M., Jo, S.J., Lee, J., Cho, S.-Y., Kim, S.-H., Lee, D.-G. and Jeong, H.-S. 2019. Clinical characteristics and antimicrobial susceptibility trends in Citrobacter bacteremia: An 11-year single-center experience. Infection and Chemotherapy, 51, 1. https://doi.org/10.3947/ic.2019.51.1.1

Levallois, P. and Villanueva, C. 2019. Drinking water quality and human health: An editorial. International Journal of Environmental Research and Public Health, 16, 631. https://doi.org/10.3390/ijerph16040631

Lin, L., Yang, H. and Xu, X. 2022. Effects of water pollution on human health and disease heterogeneity: A review. Frontiers in Environmental Sciences, 10, 880246. https://doi.org/10.3389/fenvs.2022.880246

Mokomane, M., Kasvosve, I., Melo, E.D., Pernica, J.M. and Goldfarb, D.M. 2018. The global problem of childhood diarrhoeal diseases: emerging strategies in prevention and management. Theoriticial Advances in Infectious Diseases, 5, 29-43. https://doi.org/10.1177/2049936117744429

Mulamattathil, S.G., Bezuidenhout, C., Mbewe, M. and Ateba, C.N. 2014. Isolation of environmental bacteria from surface and drinking water in Mafikeng, South Africa, and characterization using their antibiotic resistance profiles. Journal of Pathogens, 2014, 1-11. https://doi.org/10.1155/2014/371208

Sehnal, L., Brammer-Robbins, E., Wormington, A.M., Blaha, L., Bisesi, J., Larkin, I., Martyniuk, C.J., Simonin, M. and Adamovsky, O. 2021. Microbiome composition and function in aquatic vertebrates: Small organisms making big impacts on aquatic animal health. Frontiers in Microbiology, 12, 567408. https://doi.org/10.3389/fmicb.2021.567408

Shayo, G.M., Elimbinzi, E., Shao, G.N. and Fabian, C. 2023. Severity of waterborne diseases in developing countries and the effectiveness of ceramic filters for improving water quality. Bulletin of National Research Centere, 47, 113. https://doi.org/10.1186/s42269-023-01088-9

Singh, P., Saini, P., Sachan, S. and Dubey, S. 2016. Characterization, antimicrobial activity and antibiotic susceptibility of lactic acid bacteria isolated from food samples. International Journal of Current Microbiology and Applied Sciences, 5, 901-911. https://doi.org/10.20546/ijcmas.2016.507.102

Sojka, M. and Jasku³a, J. 2022. Heavy Metals in river sediments: Contamination, toxicity, and source identification - A case study from Poland. International Journal of Environmental Research and Public Health, 19, 10502. https://doi.org/10.3390/ijerph191710502

Tripathi, N. and Sapra, A. 2023. Gram Staining. StarPearls Publishing. Treasure iland, USA. https://www.ncbi.nlm. nih.gov/books/NBK562156/

Varela, M.F., Stephen, J., Lekshmi, M., Ojha, M., Wenzel, N., Sanford, L.M., Hernandez, A.J., Parvathi, A. and Kumar, S.H. 2021. Bacterial resistance to antimicrobial agents. Antibiotics, 10, 593. https://doi.org/10.3390/antibiotics10050593

Yates, M.V. 2018. Drinking water microbiology. In: Reference Module in Biomedical Sciences. Elsevier, p. B9780128012383661238. https://doi.org/10.1016/B978-0-12-801238-3.66123-8

Zaric, R.Z., Jankovic, S., Zaric, M., Milosavljevic, M., Stojadinovic, M. and Pejcic, A. 2021. Antimicrobial treatment of Morganella morganii invasive infections: Systematic review. Indian Journal of Medical Microbiology, 39, 404-412. https://doi.org/10.1016/j.ijmmb.2021.06.005

Zhang, H., Xu, L., Huang, T., Yan, M., Liu, K., Miao, Y., He, H., Li, S. and Sekar, R. 2021. Combined effects of seasonality and stagnation on tap water quality: Changes in chemical parameters, metabolic activity and co-existence in bacterial community. Journal of Hazardeous Materials, 403, 124018. https://doi.org/10.1016/j.jhazmat. 2020.124018

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Published

2024-07-15

How to Cite

Eusebius, A., Raju, M., Shukla, K., & Singh, P. (2024). Tap Water Microbial Contamination in Prayagraj: Isolation, Purification and Characterization of Pathogenic Bacteria and Their Antibiotic Susceptibility Patterns. International Journal of Ecology and Environmental Sciences. https://doi.org/10.55863/ijees.2024.0347

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